Detection of Wuchereria bancrofti in mosquitoes by the polymerase chain reaction: a potentially useful tool for large-scale control programmes.
Identifieur interne : 012E05 ( Main/Exploration ); précédent : 012E04; suivant : 012E06Detection of Wuchereria bancrofti in mosquitoes by the polymerase chain reaction: a potentially useful tool for large-scale control programmes.
Auteurs : H A Farid [Égypte] ; R E Hammad ; M M Hassan ; Z S Morsy ; I H Kamal ; G J Weil ; R M RamzySource :
- Transactions of the Royal Society of Tropical Medicine and Hygiene [ 0035-9203 ]
Descripteurs français
- KwdFr :
- ADN des helminthes (analyse), Animaux, Culex (parasitologie), Femelle, Filariose lymphatique (), Filariose lymphatique (épidémiologie), Humains, Lutte contre les moustiques (), Prévalence, Réaction de polymérisation en chaîne (), Wuchereria bancrofti (isolement et purification), Égypte (épidémiologie).
- MESH :
- analyse : ADN des helminthes.
- isolement et purification : Wuchereria bancrofti.
- parasitologie : Culex.
- épidémiologie : Filariose lymphatique, Égypte.
- Animaux, Femelle, Filariose lymphatique, Humains, Lutte contre les moustiques, Prévalence, Réaction de polymérisation en chaîne.
- Wicri :
- geographic : Égypte.
English descriptors
- KwdEn :
- Animals, Culex (parasitology), DNA, Helminth (analysis), Egypt (epidemiology), Elephantiasis, Filarial (epidemiology), Elephantiasis, Filarial (prevention & control), Female, Humans, Mosquito Control (methods), Polymerase Chain Reaction (methods), Prevalence, Wuchereria bancrofti (isolation & purification).
- MESH :
- chemical , analysis : DNA, Helminth.
- geographic , epidemiology : Egypt.
- epidemiology : Elephantiasis, Filarial.
- isolation & purification : Wuchereria bancrofti.
- methods : Mosquito Control, Polymerase Chain Reaction.
- parasitology : Culex.
- prevention & control : Elephantiasis, Filarial.
- Animals, Female, Humans, Prevalence.
Abstract
Focally endemic bancroftian filariasis is targeted for elimination in the Nile delta of Egypt. Improved methods are needed for identifying endemic villages to be included in the control programme and for monitoring its success. We have evaluated the performance of a polymerase chain reaction (PCR) assay in estimating Wuchereria bancrofti infection in pools of Culex pipiens (1-25 females) from 2 adjacent villages with high (El Qolzom, 10.8%) and low (Kafr Shorafa, 2.1%) prevalence rates of human filariasis. This assay detects a repeated sequence in W. bancrofti deoxyribonucleic acid (DNA). Mosquitoes resting within houses were captured by aspiration and pooled by house. Houses were classified as positive or negative for human filarial infection based on night blood examinations of residents. The assay detected parasite DNA in mosquitoes from 60% of 25 infected houses and 24% of 25 uninfected houses. PCR processing of mosquitoes caught within houses of unknown filariasis infection status (44 in El Qolzom, 37 in Kafr Shorafa) identified 31.8% and 8.1% of houses, respectively, as containing infected mosquitoes. These results support the validity of the PCR assay for evaluating filarial prevalence in different villages. C. pipiens collected outdoors in dry ice-baited traps and tested by PCR (266 in Qolzom, 82 in Kafr Shorafa) did not contain parasite DNA. Pools of female mosquitoes (296 in Qolzom, 240 in Kafr Shorafa) captured in oviposition traps were also negative. We concluded that the PCR based assay is a powerful epidemiological tool that can be used for evaluating W. bancrofti infection in villages in the Nile delta and for monitoring the application of control programmes in filariasis endemic areas.
PubMed: 11280059
Affiliations:
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Le document en format XML
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xml:lang="en">Focally endemic bancroftian filariasis is targeted for elimination in the Nile delta of Egypt. Improved methods are needed for identifying endemic villages to be included in the control programme and for monitoring its success. We have evaluated the performance of a polymerase chain reaction (PCR) assay in estimating Wuchereria bancrofti infection in pools of Culex pipiens (1-25 females) from 2 adjacent villages with high (El Qolzom, 10.8%) and low (Kafr Shorafa, 2.1%) prevalence rates of human filariasis. This assay detects a repeated sequence in W. bancrofti deoxyribonucleic acid (DNA). Mosquitoes resting within houses were captured by aspiration and pooled by house. Houses were classified as positive or negative for human filarial infection based on night blood examinations of residents. The assay detected parasite DNA in mosquitoes from 60% of 25 infected houses and 24% of 25 uninfected houses. PCR processing of mosquitoes caught within houses of unknown filariasis infection status (44 in El Qolzom, 37 in Kafr Shorafa) identified 31.8% and 8.1% of houses, respectively, as containing infected mosquitoes. These results support the validity of the PCR assay for evaluating filarial prevalence in different villages. C. pipiens collected outdoors in dry ice-baited traps and tested by PCR (266 in Qolzom, 82 in Kafr Shorafa) did not contain parasite DNA. Pools of female mosquitoes (296 in Qolzom, 240 in Kafr Shorafa) captured in oviposition traps were also negative. We concluded that the PCR based assay is a powerful epidemiological tool that can be used for evaluating W. bancrofti infection in villages in the Nile delta and for monitoring the application of control programmes in filariasis endemic areas.</div></front></TEI><affiliations><list><country><li>Égypte</li></country></list><tree><noCountry><name sortKey="Hammad, R E" sort="Hammad, R E" uniqKey="Hammad R" first="R E" last="Hammad">R E Hammad</name><name sortKey="Hassan, M M" sort="Hassan, M M" uniqKey="Hassan M" first="M M" last="Hassan">M M Hassan</name><name sortKey="Kamal, I H" sort="Kamal, I H" uniqKey="Kamal I" first="I H" last="Kamal">I H Kamal</name><name sortKey="Morsy, Z S" sort="Morsy, Z S" uniqKey="Morsy Z" first="Z S" last="Morsy">Z S Morsy</name><name sortKey="Ramzy, R M" sort="Ramzy, R M" uniqKey="Ramzy R" first="R M" last="Ramzy">R M Ramzy</name><name sortKey="Weil, G J" sort="Weil, G J" uniqKey="Weil G" first="G J" last="Weil">G J Weil</name></noCountry><country name="Égypte"><noRegion><name sortKey="Farid, H A" sort="Farid, H A" uniqKey="Farid H" first="H A" last="Farid">H A Farid</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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